igf2 (R&D Systems)
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igf2
Igf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igf2/Recombinant+Human+IGF-II%2FIGF2+Protein%2C+CF/us12606639-1424-14-15
Average 93 stars, based on 69 article reviews
Igf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igf2/Recombinant+Human+IGF-II%2FIGF2+Protein%2C+CF/us12606639-1424-14-15
Average 93 stars, based on 69 article reviews
igf2 - by Bioz Stars,
2026-11
93/100 stars
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Blocking Assay:Article Title: CytoSignal Detects Locations and Dynamics of Ligand-Receptor Signaling at Cellular Resolution from Spatial Transcriptomic Data Article Snippet: Samples were embedded in an OCT compound (Tissue-Tek, Sakura) and cryosectioned at 14 μm using a cryostat (Leica CM1860) and adhered to positively charged glass slides (Fisherbrand ColorFrost Plus). .. Sections were postfixed in 4% paraformaldehyde for 15 min at room temperature, treated with 0.1% TritonX-100 for 10 min at RT for permeabilization, blocked with Duolink® blocking solution(DUO82007) at 37 °C for 1 h, and incubated with each antibodies for FGF8(Goat polyclonal, 1:200, Invitrogen, PA5-47598) and FGFR1(Rabbit polyclonal, 1:200, Abcam, ab59194), or for OSTP(Goat polyclonal, 1:200 R&D system, AF808) and CD44(Rabbit polyclonal, 1:200, Proteintech,15675-1-AP), or for Incubation:Article Title: CytoSignal Detects Locations and Dynamics of Ligand-Receptor Signaling at Cellular Resolution from Spatial Transcriptomic Data Article Snippet: Samples were embedded in an OCT compound (Tissue-Tek, Sakura) and cryosectioned at 14 μm using a cryostat (Leica CM1860) and adhered to positively charged glass slides (Fisherbrand ColorFrost Plus). .. Sections were postfixed in 4% paraformaldehyde for 15 min at room temperature, treated with 0.1% TritonX-100 for 10 min at RT for permeabilization, blocked with Duolink® blocking solution(DUO82007) at 37 °C for 1 h, and incubated with each antibodies for FGF8(Goat polyclonal, 1:200, Invitrogen, PA5-47598) and FGFR1(Rabbit polyclonal, 1:200, Abcam, ab59194), or for OSTP(Goat polyclonal, 1:200 R&D system, AF808) and CD44(Rabbit polyclonal, 1:200, Proteintech,15675-1-AP), or for Article Title: Autocrine insulin-like growth factor 2 signaling as a potential target in the associated development of pulmonary emphysema and cancer in smokers Article Snippet: .. Primary antibodies were incubated overnight at 4°C with the following antibodies: Pdpn (1:500) (hamster, Developmental Studies Hybridoma Bank, Iowa City, IA, USA), SPC (1:200) (rabbit, Millipore, Billerica, MA, USA), MPO (1:100) (rabbit, Abcam, Cambridge, UK), F4/80 (1:100) (rat, Bio-Rad Laboratories, Hercules, CA, USA), CD4 (1:200) (rabbit, Abcam), CD8 (1:200) (rat, Abcam), Muc1 (1:500) (hamster, Thermo Fisher Scientific, Waltham, MA, USA), Article Title: TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. Article Snippet: .. To induce differentiation, C2C12 cells transfected with control siRNA or siRNA against TMED10 were incubated with DMEM containing 2% horse serum and 1% penicillin–streptomycin in the presence or absence of 100 ng/mL purified Negative Control:Article Title: CytoSignal Detects Locations and Dynamics of Ligand-Receptor Signaling at Cellular Resolution from Spatial Transcriptomic Data Article Snippet: Samples were embedded in an OCT compound (Tissue-Tek, Sakura) and cryosectioned at 14 μm using a cryostat (Leica CM1860) and adhered to positively charged glass slides (Fisherbrand ColorFrost Plus). .. Sections were postfixed in 4% paraformaldehyde for 15 min at room temperature, treated with 0.1% TritonX-100 for 10 min at RT for permeabilization, blocked with Duolink® blocking solution(DUO82007) at 37 °C for 1 h, and incubated with each antibodies for FGF8(Goat polyclonal, 1:200, Invitrogen, PA5-47598) and FGFR1(Rabbit polyclonal, 1:200, Abcam, ab59194), or for OSTP(Goat polyclonal, 1:200 R&D system, AF808) and CD44(Rabbit polyclonal, 1:200, Proteintech,15675-1-AP), or for Cell-Signaling:Article Title: Autocrine insulin-like growth factor 2 signaling as a potential target in the associated development of pulmonary emphysema and cancer in smokers Article Snippet: .. Primary antibodies were incubated overnight at 4°C with the following antibodies: Pdpn (1:500) (hamster, Developmental Studies Hybridoma Bank, Iowa City, IA, USA), SPC (1:200) (rabbit, Millipore, Billerica, MA, USA), MPO (1:100) (rabbit, Abcam, Cambridge, UK), F4/80 (1:100) (rat, Bio-Rad Laboratories, Hercules, CA, USA), CD4 (1:200) (rabbit, Abcam), CD8 (1:200) (rat, Abcam), Muc1 (1:500) (hamster, Thermo Fisher Scientific, Waltham, MA, USA), Enzyme-linked Immunosorbent Assay:Article Title: Circulating Adipocytokines and Insulin Like-Growth Factors and Their Modulation in Obesity-Associated Endometrial Cancer Article Snippet: .. Enzyme linked immunosorbent assay (ELISA) was performed at room temperature, as per the manufacturer’s instructions; Adiponectin (#DY1065), Leptin (#DY398), IGF1 (#DY291), Article Title: Sperm IGF2 protein levels: implications for early embryo development Article Snippet: .. Sample aliquots were stored at -20°C for assessment of mature Western Blot:Article Title: Antigen binding proteins Article Snippet: .. Detection of cleavage events was performed via Western Blot analysis as follows: IGFBP-2 and Article Title: TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. Article Snippet: .. To induce differentiation, C2C12 cells transfected with control siRNA or siRNA against TMED10 were incubated with DMEM containing 2% horse serum and 1% penicillin–streptomycin in the presence or absence of 100 ng/mL purified Transfection:Article Title: TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. Article Snippet: .. To induce differentiation, C2C12 cells transfected with control siRNA or siRNA against TMED10 were incubated with DMEM containing 2% horse serum and 1% penicillin–streptomycin in the presence or absence of 100 ng/mL purified Control:Article Title: TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. Article Snippet: .. To induce differentiation, C2C12 cells transfected with control siRNA or siRNA against TMED10 were incubated with DMEM containing 2% horse serum and 1% penicillin–streptomycin in the presence or absence of 100 ng/mL purified Purification:Article Title: TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. Article Snippet: .. To induce differentiation, C2C12 cells transfected with control siRNA or siRNA against TMED10 were incubated with DMEM containing 2% horse serum and 1% penicillin–streptomycin in the presence or absence of 100 ng/mL purified Immunofluorescence:Article Title: TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. Article Snippet: .. To induce differentiation, C2C12 cells transfected with control siRNA or siRNA against TMED10 were incubated with DMEM containing 2% horse serum and 1% penicillin–streptomycin in the presence or absence of 100 ng/mL purified Activation Assay:Article Title: Chryseobacterium indologenes mediates resistance to osimertinib by activating the IGF1R pathway in EGFR-mutant lung adenocarcinoma Article Snippet: .. To assess whether IGF1R activation mediates resistance in LACs, cells were treated with recombinant human IGF1 (R&D Systems) or Recombinant:Article Title: Chryseobacterium indologenes mediates resistance to osimertinib by activating the IGF1R pathway in EGFR-mutant lung adenocarcinoma Article Snippet: .. To assess whether IGF1R activation mediates resistance in LACs, cells were treated with recombinant human IGF1 (R&D Systems) or |
